Microbiology: A Laboratory Manual 12th Edition

Part 1 Basic Laboratory Techniques for Isolation, Cultivation, and Cultural

Experiment 1: Effectiveness of Hand Washing

Experiment 2: Culture Transfer Techniques

Experiment 3: Techniques for Isolation of Pure Cultures

Experiment 4: Cultural Characteristics of Microorganisms

Part 2 Microscopy

Experiment 5: Microscopic Examination of Stained Cell Preparations

Experiment 6:Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount

Part 3 Bacterial Staining

Experiment 7: Preparation of Bacterial Smears

Experiment 8: Simple Staining

Experiment 9: Negative Staining

Experiment 10:Gram Stain

Experiment 11: Acid-Fast Stain

Experiment 12: Differential Staining for Visualization of Bacterial Cell Structures

Part 4 Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations

Experiment 13:Nutritional Requirements: Media for the Routine Cultivation of Bacteria

Experiment 14: Use of Differential, Selective, and Enriched Media

Experiment 15: Physical Factors: Temperature

Experiment 16: Physical Factors: pH of the Extracellular Environment

Experiment 17: Physical Factors: Atmospheric Oxygen Requirements

Experiment 18: Techniques for the Cultivation of Anaerobic Microorganisms

Experiment 19: Serial Dilution-Agar Plate Procedure to Quantitate Viable Cells

Experiment 20: The Bacterial Growth Curve

Part 5 Biochemical Activities of Microorganisms

Experiment 21: Extracellular Enzymatic Activities of Microorganisms

Experiment 22: Carbohydrate Fermentation

Experiment 23: Triple Sugar-Iron Agar Test

Experiment 24: IMViC Test

Experiment 25: Hydrogen Sulfide Test

Experiment 26: Urease Test

Experiment 27: Litmus-Milk Reactions

Experiment 28: Nitrate Reduction Test

Experiment 29: Catalase Test

Experiment 30: Oxidase Test

Experiment 31: Utilization of Amino Acids

Experiment 32: Genus Identification of Unknown Bacterial Cultures

Part 6 The Protozoa

Experiment 33: Free-Living Protozoa

Experiment 34: Parasitic Protozoa

Part 7 The Fungi

Experiment 35: Cultivation and Morphology of Molds

Experiment 36: Isolation of a Soil Fungal Species

Experiment 37:Morphology, Cultural Characteristics and Reproduction

Part 8 The Viruses

Experiment 38: Cultivation and Enumeration of Bacteriophages

Experiment 39: Isolation of Coliphages from Raw Sewage

Experiment 40: Propagation of Isolated Bacteriophage Cultures

Part 9 Physical and Chemical Agents for the Control of Microbial Growth

Experiment 41: Physical Agents of Control: Moist Heat

Experiment 42: Chemical Agents of Control: Chemotherapeutic Agents

Experiment 43: Determination of Penicillin Activity in the Presence and Absence of Penicillinase

Experiment 44: Chemical Agents of Control: Disinfectants and Antiseptics

Part 10 Microbiology of Food

Experiment 45: Microbiological Analysis of Food Products: Bacterial Count

Experiment 46: Isolation of Salmonella from Raw Meat

Experiment 47: Microbial Fermentation

PART 11 Microbiology of Water

Experiment 48: Standard Qualitative Analysis of Water

Experiment 49: Quantitative Analysis of Water: Membrane Filter Method

PART 12 Microbiology of Soil

Experiment 50: Microbial Populations in Soil: Enumeration

Experiment 51: Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates

Experiment 52: Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique

PART 13 Bacterial Genetics

Experiment 53: Enzyme Induction

Experiment 54: Bacterial Conjugation

Experiment 55: Isolation of a Streptomycin-Resistant Mutant

Experiment 56: The Ames Test: A Bacterial Test System for Chemical Carcinogenicity

Experiment 57: Utilization of Bacterial Plasmids

Experiment 58: Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA

PART 14 Medical Microbiology

Experiment 59: Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries

Experiment 60: Normal Microbial Flora of the Throat and Skin

Experiment 61: Identification of Human Staphylococcal Pathogens

Experiment 62: Identification of Human Streptococcal Pathogens

Experiment 63: Identification of Streptococcus pneumoniae

Experiment 64: Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems

Experiment 65: Isolation and Presumptive Identification of Campylobacter

Experiment 66: Microbiological Analysis of Urine Specimens

Experiment 67: Microbiological Analysis of Blood Specimens

Experiment 68: Species Identification of Unknown Bacterial Cultures

PART 15 Immunology

Experiment 69: Precipitin Reaction: The Ring Test

Experiment 70: Agglutination Reaction: The Febrile Antibody Test

Experiment 71: Enzyme-Linked Immunosorbent Assay

Experiment 72: Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures

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